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Historical assignment of 72109
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To test this, several experiments can be performed.

1. Since Actin polymerization doesn’t occur at low temperature, cooling cells to 4^oC will prevent this reaction. If paramecium fails to migrate at cold temperature, it will reinforce the suggested hypothesis.
2. Testing entire cell Actin concentration using a western-blot method will enable to test the hypothesis by comparing between yeast-fed and starved paramecium. The presence of Actin in fed Paramecium and its absence in starved Paramecium will support the hypothesis.
3. Use a drug that prevents Actin polymerization (a phenomenon underlying some of the intracellular trafficking), such as Cytochalasin B (CB). Inhibition of food vacuole movement will indicate that Actin microfilamets are indeed involved in the release process.
4. Add a fluorescent monoclonal antibody against actin microfilaments and check (using a microscope) if actin is observed around the newly formed  vacuole.
5. Knock out the gene encoding actin (i.e. erase the gene by genetic engineering methods). If the release process is abolished, it proves the involvement of actin microfilaments in the release process.
6. Suggest your own ideas !! Use positive and negative controls in your experimental setup.

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Submit your answers (in Hebrew) to your teaching assistant by next week.