Now that you have established the exact time course of
cell cycle progression in synchronized cultures of mouse fibroblasts, you
are ready to determine whether substance X arrests cells at the R point
or at the G1/S transition point.
You perform the experiment illustrated to the right. |
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8. You may
draw the following conclusions (TRUE
or FALSE):
A. Exposure to substance X during G0 arrests the cells in G0. B. Exposure to substance X after the cells have crossed the R point arrests the cells before S phase. C. Cells are accumulated before S phase, because the FACS analysis was performed too soon and the cells did not have enough time to enter S phase. |
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