Now that you have established the exact time course of cell cycle progression in synchronized cultures of mouse fibroblasts, you are ready to determine whether substance X arrests cells at the R point or at the G1/S transition point. 
You perform the experiment illustrated to the right.
8. You may draw the following conclusions (TRUE or FALSE):
A. Exposure to substance X during G0 arrests the cells in G0.
B. Exposure to substance X after the cells have crossed the R point arrests the cells before S phase.
C. Cells are accumulated before  S phase, because the FACS analysis was performed too soon and the cells did not have enough time to enter S phase.
Examine your results
TREATMENT
 G1
S
G2/M
No exposure to substance X
51
41
8
Exposure to substance X during t=0-8
55
35
10
Exposure to substance X during t=8-16
88
7
5