pGEX-4T-1 Control Regions:

pGEX-4T-1 Control Regions:
* Glutathione S-transferase gene region: tac promoter: -10: 205-211; -35: 183-188; lac operator: 217-237; Ribosome binding site for GST: 244; Start codon (ATG) for GST: 258; Coding region for thrombin cleavage: 918-935
* MCS: 930-966
* Beta-lactamase gene region: Promoter: -10: 1330-1335; -35: 1307-1312; Start codon (ATG): 1377; Stop codon (TAA): 2235
* lacIq gene region: Start codon (GTG): 3318; Stop codon (TGA): 4398
* Plasmid replication region: Site of replication initiation: 2995; Region necessary for replication: 2302-2998
* Sequencing primers: 5' pGEX Sequencing Primer binds nucleotides 869-891; 3' pGEX Sequencing Primer binds nucleotides 1041-1019

pGEX-4T-2 Control Regions:
* Glutathione S-transferase gene region: tac promoter: -10: 205-211; -35: 183-188; lac operator: 217-237; Ribosome binding site for GST: 244; Start codon (ATG) for GST: 258; Coding region for thrombin cleavage: 918-935
* MCS: 930-967
* Beta-lactamase gene region: Promoter: -10: 1331-1336; -35: 1308-1313; Start codon (ATG): 1378; Stop codon (TAA): 2236
* lacIq gene region: Start codon (GTG): 3319; Stop codon (TGA): 4399
* Plasmid replication region: Site of replication initiation: 2996; Region necessary for replication: 2303-2999
* Sequencing primers: 5' pGEX Sequencing Primer binds nucleotides 869-891; 3' pGEX Sequencing Primer binds nucleotides 1042-1020

pGEX-4T-3 Control Regions:
* Glutathione S-transferase gene region: tac promoter: -10: 205-211; -35: 183-188; lac operator: 217-237; Ribosome binding site for GST: 244; Start codon (ATG) for GST: 258; Coding region for thrombin cleavage: 918-935
* MCS: 930-965
* Beta-lactamase gene region: Promoter: -10: 1329-1334; -35: 1306-1311; Start codon (ATG): 1376; Stop codon (TAA): 2234
* lacIq gene region: Start codon (GTG): 3317; Stop codon (TGA): 4397
* Plasmid replication region: Site of replication initiation: 2994; Region necessary for replication: 2301-2997
* Sequencing primers: 5' pGEX Sequencing Primer binds nucleotides 869-891; 3' pGEX Sequencing Primer binds nucleotides 1040-1018