Lecture Notes

^{The Hebrew University of Jerusalem}
^{The Alexander Silberman Institute of Life Sciences}
Lecture Notes

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2005 (72682) 2006-2007 (72681/2) 2008-2009 (72681)			2009-2010 (72681)

2009-2010

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Date	Topic	Lecturer
20/10/09	Cloning and DNA manipulation techniques	Dr. Tsafi Danieli
27/10/09	Introduction to Protein Expression and Expression Systems	Dr. Tsafi Danieli
3/11/09	Bacterial Expression Systems	Dr. Tsafi Danieli
10/11/09	Cell-free Production of Proteins	Dr. Tsafi Danieli
17/11/09	Insect-based Expression Systems	Dr. Tsafi Danieli
24/11/09	Mammalian Expression Systems	Dr. Tsafi Danieli
1/12/09	Protein Purification Strategies I	Dr. Mario Lebendiker
8/12/09	Ion Exchange Chromatography Gel Filtration Chromatography	Dr. Mario Lebendiker
15/12/09	Affinity Chromatography	Dr. Mario Lebendiker
22/12/09	Hydrophobic Exchange Chromatography Other Purification Techniques Refolding and Aggregation problems	Dr. Mario Lebendiker
29/12/09	Protein Purification Strategies II - Purification Examples	Dr. Mario Lebendiker
5/01/10	Purification of Membrane Proteins Sample Preparation for X-Ray and NMR studies	Dr. Mario Lebendiker

2008-2009

**PLEASE NOTE:** Due to copy rights limitations, lecture notes may only be viewed by providing your username
(8 Digits ID) and passward (4 digits) or by using an OTP card, if you have one. (For OTP information click here)
Date	Topic	Lecturer
4/11/08	Cloning and DNA manipulation techniques	Dr. Tsafi Danieli
11/11/08	Introduction to Protein Expression and Expression Systems	Dr. Tsafi Danieli
18/11/08	Bacterial Expression Systems	Dr. Tsafi Danieli
25/11/08	Cell-free Production of Proteins	Dr. Tsafi Danieli
2/12/08	Insect-based Expression Systems	Dr. Tsafi Danieli
9/12/08	Mammalian Expression Systems	Dr. Tsafi Danieli
16/12/08	Protein Purification Strategies I	Dr. Mario Lebendiker
23/12/08	Ion Exchange Chromatography Gel Filtration Chromatography	Dr. Mario Lebendiker
30/12/08	Affinity Chromatography	Dr. Mario Lebendiker
6/01/09	Hydrophobic Exchange Chromatography Other Purification Techniques Refolding and Aggregation problems	Dr. Mario Lebendiker
13/01/09	Protein Purification Strategies II - Purification Examples	Dr. Mario Lebendiker
20/01/09	Purification of Membrane Proteins Sample Preparation for X-Ray and NMR studies	Dr. Mario Lebendiker

2006-2007
**PLEASE NOTE:** Due to copy rights limitations, lecture notes may only be viewed by providing your username
(8 Digits ID) and passward (4 digits) or by using an OTP card, if you have one. (For OTP information click here)
Date	Topic	Lecturer
23/10/06	Introduction to Protein Expression and Expression Systems	Dr. Tsafi Danieli
30/10/06	Bacterial Expression Systems	Dr. Tsafi Danieli
06/11/06	Insect-based Expression Systems	Dr. Tsafi Danieli
13/11/06	Mammalian Expression Systems	Dr. Tsafi Danieli
20/11/06	In-Vitro Production of Proteins	Dr. Tsafi Danieli
27/11/06	Protein Purification Strategies I	Dr. Mario Lebendiker
04/12/06	Ion Exchange Chromatography Gel Filtration Chromatography	Dr. Mario Lebendiker
11/12/06	Affinity Chromatography	Dr. Mario Lebendiker
18/12/06	Hydrophobic Exchange Chromatography Other Purification Techniques Refolding	Dr. Mario Lebendiker
25/12/06	Protein Purification Strategies II - Purification Examples Sample Preparation for X-Ray and NMR studies	Dr. Mario Lebendiker
01/01/07	Membrane Proteins	Dr. Mario Lebendiker
08/01/07	Using bioinformatics tools - background Using bioinformatics tools - case study	Dr. Nurit Doron
15/01/07	In-Vitro Production of Proteins	Dr. Tsafi Danieli
22/01/07	Expression in Plant Cells	Dr. Yossi Shaaltiel

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2005
**PLEASE NOTE:** Due to copy rights limitations, lecture notes may only be viewed by providing your username
(8 Digits ID) and passward (4 digits) or by using an OTP card, if you have one. (For OTP information click here)
Date	Topic	Lecturer
19/06/05	Introduction to Protein Expression	Dr. Tsafi Danieli
19/06/05	Introduction to Expression Systems	Dr. Tsafi Danieli
20/06/05	Protein Purification Strategies I	Dr. Mario Lebendiker
20/06/05	Gel Filtration	Dr. Mario Lebendiker
21/06/05	The Bacterial Expression Systems	Dr. Tsafi Danieli
21/06/05	The Bacterial Expression Systems	Dr. Tsafi Danieli
22/06/05	Ion Exchange Chromatography	Dr. Mario Lebendiker
22/06/05	Hydrophobic Exchange	Dr. Mario Lebendiker
23/06/05	Refolding	Dr. Mario Lebendiker
23/06/05	Construction and Work of Industrial Fermenters	Prof.. Sergei Brawn
26/06/05	Affinity Chromatography	Dr. Mario Lebendiker
26/06/05	Affinity Chromatography	Dr. Mario Lebendiker
27/06/05	Insect-based Expression Systems	Dr. Tsafi Danieli
27/06/05	Insect-based Expression Systems	Dr. Tsafi Danieli
28/06/05	Other Purification Techniques	Dr. Mario Lebendiker
28/06/05	Protein Purification Strategies II - Purification Examples	Dr. Mario Lebendiker
29/06/05	Mammalian Expression Systems	Dr. Tsafi Danieli
29/06/05	In-Vitro Production of Proteins	Dr. Tsafi Danieli
30/06/05	Sample Preparation for X-Ray and NMR studies	Dr. Mario Lebendiker
30/06/05	Luminescent and Fluorescence Protein Detection Methods	Dr. Keith V. Wood - (Promega Corporation)

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