2. Right - the Rosetta-gami bacterial strain allows formation of S-S bonds in the cytoplasm and supplies several rare tRNAs. Our computational analysis suggests the existence of these problems, so using this strain may help. However, this strain grows very slowly and is capable of lower capacity of protein synthesis than the Origami and Rosetta bacterial strains. Thus it may be a good idea to coduct a minimal screen using all 3 strains.

3. Right - the computational analysis suggests the existence of a signal peptide that is cleaved between positions 34 and 35. Thus, a truncated form may be more soluble, when expressed in bacteria. If we want to express this protein in order to manufacture antibodies, antibodies can be generated against proteins found in inclusion bodies. However, a truncated form of the protein might impair its activity.

4. Wrong - nice try though. No bacterial strain is capabale of glycosylation.

5. Wrong - these sites are predicted to undergo phosphorylation. However, NetPhos performs these predictions for eukaryotic proteins. The same proteins may undergo different phosphorylation patterns in bacteria.

7. Wrong - this is too early to loose hope!!

6. Right - bacterial strains that contain more membranes may lead to better expression of proteins containing transmembrane domains, reduce cytotoxic effects of these proteins, and reduce inclusion bodies formation.