1. False - a band of ~55KDa, which we suspect is GFP-GST is produced in large quantities. However, additional products of smaller size are also observed. This may indicate a small degree of proteolytic cleavage or some problems of premature termination.
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2. True - most of the products are found in the pellet.

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3. True

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4. False - these products are also observed in the GST-GFP control sample. They are NOT specific products of NS4B!

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5. True - however at 37oc we observe high amounts of smaller protein products.

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6. False - at two hours post induction, samples grown in 37oc (but not in room temperature) show such differences. Product is observed only when 1mM is used. As opposed to that, under all other experimental conditions no differences are observed following use of the various IPTG concentrations.

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