Input Sequence
QUERY (261 aa)
ASHLPYIEQG MQLAEQFKQK AIGLLQTATK QAEAAAPVVE SKWRTLEAFW
AKHMWNFISG IQYLAGLSTL PGNPAIASLM AFTASITSPL TTQHTLLFNI
LGGWVAAQLA PPSAASAFVG AGIAGAAVGS IGLGKVLVDI LAGYGAGVAG
ALVAFKVMSG EMPSTEDLVN LLPAILSPGA LVVGVVCAAI LRRHVGPGEG
AVQWMNRLIA FASRGNHVSP THYVPESDAA ARVTQILSSL TITQLLKRLH
QWINEDCSTP C
Results of Subprograms
*** Warning: 1st aa is not methyonine
PSG: a new signal peptide prediction method
N-region: length 8; pos.chg 0; neg.chg 1
H-region: length 6; peak value 0.00
PSG score: -4.40
GvH: von Heijne's method for signal seq. recognition
GvH score (threshold: -2.1): -6.21
possible cleavage site: between 34 and 35
>>> Seems to have no N-terminal signal peptide
ALOM: Klein et al's method for TM region allocation
Init position for calculation: 1
Tentative number of TMS(s) for the threshold 0.5: 3
Number of TMS(s) for threshold 0.5: 1
INTEGRAL Likelihood = -7.96 Transmembrane 175 - 191
PERIPHERAL Likelihood = 2.38 (at 75)
ALOM score: -7.96 (number of TMSs: 1)
MTOP: Prediction of membrane topology (Hartmann et al.)
Center position for calculation: 182
Charge difference: 3.5 C( 1.5) - N(-2.0)
C > N: C-terminal side will be inside
>>> membrane topology: type 1b (cytoplasmic tail 175 to 261)
MITDISC: discrimination of mitochondrial targeting seq
R content: 0 Hyd Moment(75): 5.09
Hyd Moment(95): 1.85 G content: 1
D/E content: 2 S/T content: 1
Score: -7.86
Gavel: prediction of cleavage sites for mitochondrial preseq
cleavage site motif not found
NUCDISC: discrimination of nuclear localization signals
pat4: none
pat7: none
bipartite: none
content of basic residues: 5.7%
NLS Score: -0.47
KDEL: ER retention motif in the C-terminus: none
ER Membrane Retention Signals: none
SKL: peroxisomal targeting signal in the C-terminus: none
SKL2: 2nd peroxisomal targeting signal: none
VAC: possible vacuolar targeting motif: none
RNA-binding motif: none
Actinin-type actin-binding motif:
type 1: none
type 2: none
NMYR: N-myristoylation pattern : none
Prenylation motif: none
memYQRL: transport motif from cell surface to Golgi: none
Tyrosines in the tail: too long tail
Dileucine motif in the tail: found
LL at 245
checking 63 PROSITE DNA binding motifs: none
checking 71 PROSITE ribosomal protein motifs: none
checking 33 PROSITE prokaryotic DNA binding motifs: none
NNCN: Reinhardt's method for Cytplasmic/Nuclear discrimination
Prediction: cytoplasmic
Reliability: 94.1
COIL: Lupas's algorithm to detect coiled-coil regions
total: 0 residues
k = 9/23
39.1 %: cytoplasmic
17.4 %: mitochondrial
17.4 %: nuclear
13.0 %: endoplasmic reticulum
4.3 %: Golgi
4.3 %: vesicles of secretory system
4.3 %: peroxisomal
>> prediction for QUERY is cyt (k=23)