Input Sequence

QUERY (261 aa) 

ASHLPYIEQG MQLAEQFKQK AIGLLQTATK QAEAAAPVVE SKWRTLEAFW
AKHMWNFISG IQYLAGLSTL PGNPAIASLM AFTASITSPL TTQHTLLFNI
LGGWVAAQLA PPSAASAFVG AGIAGAAVGS IGLGKVLVDI LAGYGAGVAG
ALVAFKVMSG EMPSTEDLVN LLPAILSPGA LVVGVVCAAI LRRHVGPGEG
AVQWMNRLIA FASRGNHVSP THYVPESDAA ARVTQILSSL TITQLLKRLH
QWINEDCSTP C


Results of Subprograms

*** Warning: 1st aa is not methyonine

PSG:  a new signal peptide prediction method
      N-region:  length 8;  pos.chg 0;  neg.chg 1
      H-region:  length 6;  peak value   0.00
      PSG score:  -4.40

GvH:  von Heijne's method for signal seq. recognition
      GvH score (threshold: -2.1):  -6.21
      possible cleavage site: between 34 and 35

>>> Seems to have no N-terminal signal peptide

ALOM: Klein et al's method for TM region allocation
      Init position for calculation: 1
      Tentative number of TMS(s) for the threshold  0.5:   3
      Number of TMS(s) for threshold  0.5:   1
      INTEGRAL    Likelihood = -7.96   Transmembrane  175 - 191
      PERIPHERAL  Likelihood =  2.38 (at 75)
      ALOM score:  -7.96  (number of TMSs: 1)

MTOP: Prediction of membrane topology (Hartmann et al.)
      Center position for calculation: 182
      Charge difference:  3.5   C( 1.5) - N(-2.0)
      C > N: C-terminal side will be inside

>>> membrane topology: type 1b  (cytoplasmic tail 175 to 261)

MITDISC: discrimination of mitochondrial targeting seq
      R content:       0       Hyd Moment(75):  5.09
      Hyd Moment(95):  1.85    G content:       1
      D/E content:     2       S/T content:     1
      Score: -7.86

Gavel: prediction of cleavage sites for mitochondrial preseq
      cleavage site motif not found

NUCDISC: discrimination of nuclear localization signals
      pat4: none
      pat7: none
      bipartite: none
      content of basic residues:   5.7%
      NLS Score: -0.47

KDEL: ER retention motif in the C-terminus: none

ER Membrane Retention Signals: none

SKL: peroxisomal targeting signal in the C-terminus: none

SKL2: 2nd peroxisomal targeting signal:  none

VAC: possible vacuolar targeting motif: none

RNA-binding motif: none

Actinin-type actin-binding motif:
      type 1: none
      type 2: none

NMYR: N-myristoylation pattern : none

Prenylation motif: none

memYQRL: transport motif from cell surface to Golgi: none

Tyrosines in the tail: too long tail

Dileucine motif in the tail: found
      LL at 245

checking 63 PROSITE DNA binding motifs:  none

checking 71 PROSITE ribosomal protein motifs:  none

checking 33 PROSITE prokaryotic DNA binding motifs:  none

NNCN: Reinhardt's method for Cytplasmic/Nuclear discrimination
      Prediction: cytoplasmic
      Reliability: 94.1

COIL: Lupas's algorithm to detect coiled-coil regions
      total: 0 residues 


Results of the k-NN Prediction

k = 9/23
         39.1 %: cytoplasmic
         17.4 %: mitochondrial
         17.4 %: nuclear
         13.0 %: endoplasmic reticulum
          4.3 %: Golgi
          4.3 %: vesicles of secretory system
          4.3 %: peroxisomal

>> prediction for QUERY is cyt (k=23)