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2. Wrong - Indeed the virus growth caused significant insect cells lysis, but the viri in the medium are still alive, and the virus titer is high. You will have to perform a plaque assay to determine pfu for further infections. If you are interested in obtaining viral stocks, harvesting 72 hrs post-infection is suitable.
3. Wrong - Indeed the virus growth caused significant insect cells lysis 72 hrs post-infection. However, by this time the BHR1 protein could be cleaved by proteases liberated into the medium following cell lysis. In order to determine the best harvesting point for obtaining high BHR1 protein levels, you have to determine protein levels at each time point by western Blot.
4. Wrong - at 24 hrs post-infection only a small portion of the cells are infected and can produce the BHR1 protein . Thus only small amounts of proteins will be collected. It is best to perform a western blot analysis or an activity assay in order to determine the best time for protein harvesting. However, if you don't perform such assays, a cell death of 40-50% is a reasonable indication for harvest.
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