After harvesting the virus from the transfected cells
you should determine the viral titer by performing a plaque assay
(
).
|
To the right you can see a part of a six-wells plate,
seeded with SF9 cells and containing ~25 plaques (corresponding to plate
A at dilution 10-7 ).
|
|
You did that and obtained
the following results:
|
Dilution
|
10-6
|
10-7
|
10-8
|
|
Plate A
|
Fused Plaques
|
25
|
2
|
|
Plate B
|
Fused Plaques
|
30
|
1
|
|
Plate C
|
Fused Plaques
|
27
|
28
|
what is your viral titer? 
1. There
are too many plaques - all we can say is that we have a high titer and
we should be happy.
2. 2.7x108
3. Triplicates
don't work too well - a higher viral titer was found in plate C, although
the same viral stock was used as in plates A and B.
4. 1.5x108
Did you try working with the Tetracyclin
Induced Expression System?