2. RIGHT! Glycosylation is a post-translational modification required for the proper function of the protein.
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3. WRONG! The receptor is a homotetramer, meaning that there is only one type of subunit encoded by one gene.
4. Possible, but not too promising! The receptor is indeed suspected of being toxic to neurons, but over expressing it in fibroblasts may not overcome this problem, since it is possible that by over expressing it in fibroblasts those fibroblasts will not survive.
5. RIGHT! The inducible expression system will allow the cells containing the BHR1 cDNA to grow as long as BHR1 expression is not induced. Upon induction, assays could be conducted in a short window of opportunities : after the protein appears and before cells start dying.
6. WRONG! Bacterial cells cannot support the required glycosylation/s. The protein will be expressed but will be non-functional.