1. WRONG! Bacterial cells don't support the required post-translational modifications. Proteins are likely to misfold and be inactive due to lack of glycosylation.
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2. Possible - however, you might encounter over-glycosylation (high mannose).

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3. Possible - this is our system of choice since it supports the appropriate post-translational modifications and yields high amounts of the expressed protein.

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4. Possible - this system supports appropriate post translational modifications, however the protein yield will probably be lower than that obtained using baculovirus infection of insect cells.

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5. Possible - however the protein yield will be very low and the cost of preparing the protein in this system will be too high.

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6. Possible - this system supports appropriate post translational modifications however the protein yield might be low, and the whole process is more complicated and time consuming.

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