ProtParam tool
User-provided sequence:
1 11 21 31 41 51
| | | | | |
1 MASLCSNSSS TSLKTPFTSS TTCLSSTPTA SQLFLHGKRN KTFKVSCKVT NTNGNQDETN 60
61 SVDRRNVLLG LGGLYGVANA IPLAASAAPT PPPDLSSCNK PKINATTEVP YFCCAPKPDD 120
121 MSKVPYYKFP SVTKLRIRPP AHALDEAYIA KYNLAISRMK DLDKTQPDNP IGFKQQANIH 180
181 CAYCNGGYSI DGKVLQVHNS WLFFPFHRWY LYFYERILGS LIDDPTFGLP FWNWDHPKGM 240
241 RFPPMFDVPG TALYDERRGD QIHNGNGIDL GYFGDQVETT QLQLMTNNLT LMYRQLVTNS 300
301 PCPLMSLVDL TLFGSTVEDA GTVENIPHSP VHIWVGTRRG SVLPVGKISN GEDMGNFYSA 360
361 GLDPLFYCHH SNVDRMWNEW KATGGKRTDI QNKDWLNSEF FFYDENGNPF KVRVRDCLDT 420
421 KKMGYDYHAT ATPWRNFKPK TKASAGKVNT GSIPPESQVF PLAKLDKAIS FSINRPASSR 480
481 TQQEKNAQEE VLTFNAIKYD NRDYIRFDVF LNVDNNVNAN ELDKAEFAGS YTSLPHVHRV 540
541 GDPKHTATAT LRLAITELLE DIGLEDEDTI AVTLVPKKGD ISIGGVEIKL AIVKLVCVVN 600
601 LLTLQLNKDR FCYDSVFVCW FVCLFFNFHV
References and documentation are available.
Number of amino acids: 630
Molecular weight: 70616.2
Theoretical pI: 7.21
Amino acid composition:
Ala (A) 38 6.0%
Arg (R) 26 4.1%
Asn (N) 44 7.0%
Asp (D) 42 6.7%
Cys (C) 15 2.4%
Gln (Q) 17 2.7%
Glu (E) 23 3.7%
Gly (G) 39 6.2%
His (H) 16 2.5%
Ile (I) 28 4.4%
Leu (L) 56 8.9%
Lys (K) 39 6.2%
Met (M) 11 1.7%
Phe (F) 35 5.6%
Pro (P) 40 6.3%
Ser (S) 42 6.7%
Thr (T) 44 7.0%
Trp (W) 10 1.6%
Tyr (Y) 23 3.7%
Val (V) 42 6.7%
Asx (B) 0 0.0%
Glx (Z) 0 0.0%
Xaa (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 65
Total number of positively charged residues (Arg + Lys): 65
Atomic composition:
Carbon C 3170
Hydrogen H 4868
Nitrogen N 850
Oxygen O 931
Sulfur S 26
Formula: C3170H4868N850O931S26
Total number of atoms: 9845
Extinction coefficients:
Conditions: 6.0 M guanidium hydrochloride
0.02 M phosphate buffer
pH 6.5
Extinction coefficients are in units of M-1 cm-1 .
The first table lists values computed assuming ALL Cys
residues appear as half cystines, whereas the second table
assumes that NONE do.
276 278 279 280 282
nm nm nm nm nm
Ext. coefficient 88365 89089 88375 87180 84440
Abs 0.1% (=1 g/l) 1.251 1.262 1.251 1.235 1.196
276 278 279 280 282
nm nm nm nm nm
Ext. coefficient 87350 88200 87535 86340 83600
Abs 0.1% (=1 g/l) 1.237 1.249 1.240 1.223 1.184
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 37.96
This classifies the protein as stable.
Aliphatic index: 77.37
Grand average of hydropathicity (GRAVY): -0.324
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