Query Information
ORIGIN plant
BEGIN
>EMA
MASLCSNSSS TSLKTPFTSS TTCLSSTPTA SQLFLHGKRN KTFKVSCKVT
NTNGNQDETN SVDRRNVLLG LGGLYGVANA IPLAASAAPT PPPDLSSCNK
PKINATTEVP YFCCAPKPDD MSKVPYYKFP SVTKLRIRPP AHALDEAYIA
KYNLAISRMK DLDKTQPDNP IGFKQQANIH CAYCNGGYSI DGKVLQVHNS
WLFFPFHRWY LYFYERILGS LIDDPTFGLP FWNWDHPKGM RFPPMFDVPG
TALYDERRGD QIHNGNGIDL GYFGDQVETT QLQLMTNNLT LMYRQLVTNS
PCPLMSLVDL TLFGSTVEDA GTVENIPHSP VHIWVGTRRG SVLPVGKISN
GEDMGNFYSA GLDPLFYCHH SNVDRMWNEW KATGGKRTDI QNKDWLNSEF
FFYDENGNPF KVRVRDCLDT KKMGYDYHAT ATPWRNFKPK TKASAGKVNT
GSIPPESQVF PLAKLDKAIS FSINRPASSR TQQEKNAQEE VLTFNAIKYD
NRDYIRFDVF LNVDNNVNAN ELDKAEFAGS YTSLPHVHRV GDPKHTATAT
LRLAITELLE DIGLEDEDTI AVTLVPKKGD ISIGGVEIKL AIVKLVCVVN
LLTLQLNKDR FCYDSVFVCW FVCLFFNFHV
Result Information
PSORT --- Prediction of Protein Localization Sites
version 6.4(WWW)
EMA 630 Residues
Species classification: 5
*** Reasoning Step: 1
Preliminary Calculation of ALOM (threshold: 0.5)
count: 3
Position of the most N-terminal TMS: 67 at i=3
MTOP: membrane topology (Hartmann et al.)
I(middle): 74 Charge diffirence(C-N): 1.0
McG: Examining signal sequence (McGeoch)
Length of UR: 12
Peak Value of UR: 0.51
Net Charge of CR: 0
Discriminant Score: -10.96
GvH: Examining signal sequence (von Heijne)
Signal Score (-3.5): -1.66
Possible cleavage site: 32
>>> Seems to have no N-terminal signal seq.
Amino Acid Composition of Predicted Mature Form:
calculated from 1
ALOM new cnt: 1 ** thrshld changed to -2
Cleavable signal was detected in ALOM?: 0B
ALOM: finding transmembrane regions (Klein et al.)
count: 1 value: -5.41 threshold: -2.0
INTEGRAL Likelihood = -5.41 Transmembrane 588 - 604 ( 585 - 607)
PERIPHERAL Likelihood = -0.53
modified ALOM score: 1.18
>>> Seems to be a Type Ib (Nexo Ccyt) membrane protein
The cytoplasmic tail is from 605 to 630 (27 Residues)
Gavel: Examining the boundary of mitochondrial targeting seq.
motif at: 41
KRNKTF
Discrimination of mitochondrial target seq.:
positive ( 2.73)
Rule: mitochondrial protein
Rule: mitochondrial protein
Rule: mitochondrial protein
Rule: mitochondrial protein
Hydrophobic moment analysis for chloroplast proteins
Hmax: 12.32 at (41)
Disc.Score from Amino Acid Composition (chloroplast)
score from the 3-11 region: 2.93
score from the 1-31 region: 16.78
Chloroplast protein? Status: positive ( 8.44)
Res.2 is Ala (a feature for chloroplast seq.)
Res.2 is Ala (a feature for chloroplast seq.)
Res.2 is Ala (a feature for chloroplast seq.)
*** Reasoning Step: 2
> Relative position of the end of the tail: 96%
KDEL Count: 0
Checking apolar signal for intramitochondrial sorting
(Gavel position 41) from: 67 to: 88 Score: 11.0
>>> Seems to have an intramitochondrial signal
Mitochondrial inner membrane? Score: 0.71
Mitochondrial intermemb.space? Score: 0.22
Checking apolar signal for intrachloroplastic sorting
found at (67-88): 11.0
Howe: Checking the consensus for intrachloropl.sorting
Precursor 1-87 Score: 52428.81
Chloroplast thylakoid memb.? Score: 0.651
SKL motif (signal for peroxisomal protein):
pos: 463(630), count: 1 AKL
SKL score (peroxisome): 0.3
Amino Acid Composition Tendency for Peroxisome: 1.64
Peroxisomal proteins? Status: notclr
AAC score (peroxisome): 0.285
Amino acid composition tendency for vacuolar proteins
Score: -0.61 Status: negative
Checking the amount of Basic Residues (nucleus)
Checking the 4 residue pattern for Nuclear Targeting
Checking the 7 residue pattern for Nuclear Targeting
Checking the Robbins & Dingwall consensus (nucleus)
Checking the RNA binding motif (nucleus or cytoplasm)
Nuclear Signal Status: negative ( 0.00)
Check cytoplasmic tail for typeIb (plasma memb.)
Checking the NPXY motif..
Checking the YXRF motif..
Checking N-myristoylation..
----- Final Results -----
chloroplast thylakoid membrane --- Certainty= 0.930(Affirmative) < succ>
mitochondrial inner membrane --- Certainty= 0.818(Affirmative) < succ>
chloroplast stroma --- Certainty= 0.800(Affirmative) < succ>
chloroplast thylakoid space --- Certainty= 0.800(Affirmative) < succ>
----- The End -----