Query Information

ORIGIN plant
BEGIN
>MYSEQ
 MASLCSNSSS TSLKTPFTSS TTCLSSTPTA SQLFLHGKRN KTFKVSCKVT
 NTNGNQDETN SVDRRNVLLG LGGLYGVANA IPLAASAAPT PPPDLSSCNK
 PKINATTEVP YFCCAPKPDD MSKVPYYKFP SVTKLRIRPP AHALDEAYIA
 KYNLAISRMK DLDKTQPDNP IGFKQQANIH CAYCNGGYSI DGKVLQVHNS
 WLFFPFHRWY LYFYERILGS LIDDPTFGLP FWNWDHPKGM RFPPMFDVPG
 TALYDERRGD QIHNGNGIDL GYFGDQVETT QLQLMTNNLT LMYRQLVTNS
 PCPLMSLVDL TLFGSTVEDA GTVENIPHSP VHIWVGTRRG SVLPVGKISN
 GEDMGNFYSA GLDPLFYCHH SNVDRMWNEW KATGGKRTDI QNKDWLNSEF
 FFYDENGNPF KVRVRDCLDT KKMGYDYHAT ATPWRNFKPK TKASAGKVNT
 GSIPPESQVF PLAKLDKAIS FSINRPASSR TQQEKNAQEE VLTFNAIKYD
 NRDYIRFDVF LNVDNNVNAN ELDKAEFAGS YTSLPHVHRV GDPKHTATAT
 LRLAITELLE DIGLEDEDTI AVTLVPKKGD ISIGGVEIKL AIVKLVCVVN
 LLTLQLNKDR FCYDSVFVCW FVCLFFNFHV 

Result Information

PSORT --- Prediction of Protein Localization Sites
                                            version 6.4(WWW)
MYSEQ          423 Residues
Species classification: 4

*** Reasoning Step: 1

Preliminary Calculation of ALOM (threshold: 0.5)
     count: 0
McG: Examining signal sequence (McGeoch)
     Length  of  UR:   7
     Peak Value of UR:  -0.08
     Net Charge of CR: -1
     Discriminant Score:    -17.26
GvH: Examining signal sequence (von Heijne)
     Signal Score (-3.5): -2.4
     Possible cleavage site: 39
>>> Seems to have no N-terminal signal seq.
Amino Acid Composition of Predicted Mature Form:
   calculated from 1
ALOM new cnt: 0 ** thrshld changed to -2
Cleavable signal was detected in ALOM?: 0B
ALOM: finding transmembrane regions (Klein et al.)
     count: 0  value:   4.24  threshold: -2.0
     PERIPHERAL  Likelihood =  4.24
     modified ALOM score:  -1.75
( 1) or uncleavable?
Gavel: Examining the boundary of mitochondrial targeting seq.
      motif at: 1
     Uncleavable? Ipos set to: 11
Discrimination of mitochondrial target seq.: 
     negative (-4.17)

*** Reasoning Step: 2

KDEL   Count: 0
Checking apolar signal for intramitochondrial sorting
Mitochondrial matrix?  Score:  0.10
SKL motif (signal for peroxisomal protein): 
     pos: 137(423), count: 2   AHL
     SKL score (peroxisome):  0.3
Amino Acid Composition Tendency for Peroxisome:   1.05
Peroxisomal proteins?   Status: notclr
     AAC score (peroxisome): 0.207
Amino Acid Composition tendency for lysosomal proteins
     score: -1.08  Status: negative
Modified score for lysosome: 0.100
Checking the amount of Basic Residues (nucleus)
Checking the 4 residue pattern for Nuclear Targeting
Checking the 7 residue pattern for Nuclear Targeting
Checking the Robbins & Dingwall consensus (nucleus)
Checking the RNA binding motif (nucleus or cytoplasm)
Nuclear Signal   Status: negative ( 0.00)
Checking CaaX motif..
Checking N-myristoylation..
Checking CaaX motif..
Cytoplasmic protein?   Score1: 0.450

----- Final Results -----

                        cytoplasm --- Certainty= 0.450(Affirmative) < succ>
           microbody (peroxisome) --- Certainty= 0.445(Affirmative) < succ>
       mitochondrial matrix space --- Certainty= 0.100(Affirmative) < succ>
                 lysosome (lumen) --- Certainty= 0.100(Affirmative) < succ>


----- The End -----