Written by: Erez Podoly, Tsafi Danielli, Nurit Kleinberger-Doron
Cloning is known to be a long and
frustrating procedure. Usually, the rate limiting step in traditional cloning
procedures is the ligation reaction. Recently, a few companies developed
an alternative way for cloning which replaces ligation with recombination
(as you'll soon see). We'll introduce here the system developed by Gibco-BRL:
Gateway Cloning System.
The purpose of this tutorial is to let you experiment with this method and learn to avoid possible problems associated with it.You will perform various virtual experiments. Each experiment will be followed by several multiple choice questions. The different answers will lead you to additional comments and explanations. Wrong answers can lead you to valuable comments and insights, so don't rush through the tutorial.