1. Wrong No BP entry clone was involved in this reaction. The engative control contains only the pDest vector without the Entry clone.
    2. Check the other possibilities

































  2. Most likely. Some Destination Vectors have an inherently higher background than others, possibly due to tendency to mutations or deletions in the ccdB gene. This will allow the growth of bacterial colonies containing a less toxic ccdB gene.
    3. Check the other possibilities






























  3. Unlikely. The small colonies on the plate are the result of pDest vectors that didn't undergo recombination. These colonies contain the original vector which also showed as small colonies on the negative control plates.
    4. Check the other possibilities



     
     
     
     
     
     
     
     
     
     
     
     
     
     
     
     
     
     
     
     
     
     
     
     
     
     
     
     
     
     
     
     
     
     
     
     

  4. Most Likely. Varify the result by restriction analysis with apropriate restriction enzymes.
Check the other possibilities
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 

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