|
|
|
|
|
|
 |
 |
You have picked 4 colonies from the ras reaction plate:
2 large colonies and 2 small ones. You have also picked a colony from the
negative control plate. After an overnight growth and mini-scale DNA preparation 
you have restricted the DNA from all 5 colonies with NcoI.(Nco
I cuts once inside the ras sequence within pDest14Ras. It also cuts once
within pDest14 in the part that excises from the plasmid after recombination
with ras. Thus NcoI linearizes both plasmids.) You
ran the restriction products on a 1% agarose gel. Here are your results:
Click on the numbers of lanes containing the ras gene inside the expression vector.