Sequencing of
the gene has allowed us to determine the primary amino acid composition
of the enzyme. Our first task is to determine the physical characteristics
of the protein in order to develop a purification strategy. The amino acid
sequence of the enzyme Macho-ase, is given below in Fasta format:
mgkvkvgvngfgrigrlvtraafnsgkvdivaindpfidlnymvymfqydsthgkfhgtvkaengklvin
gnpitifqerdpskikwgdagaeyvvestgvfttmekagahlqggakrviisapsadapmfvmgvnheky
dnslkiisnascttnclaplakvihdnfgiveglmttvhaitatqktvdgpsg
Click here
to open a second window showing the “Compute pI/MW tool”
Copy the sequence
above into the appropriate box in the “Compute” window.
Now press the button
“click here to compute” at the bottom of the "Compute" window.
The calculated molecular
weight and pI for Macho-ase are:
-
8.49
/ 200662.69
-
8.49
/ 20662.69
-
4.89
/ 20662.69