From: http://www.promega.com/figures/frame.asp?fn=0286VC
What is T7?
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The
cleavage site of T7ase which is located at the begining of the multiple
cloning site.
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The
T7 polymerase promoter just prior to the multiple cloning site.
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A
reporter gene which is disrupted if a fragment of DNA is inserted into
the multiple cloning site.
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The
beginning of the origin of replication.