1. Wrong - technically you can perform the cloning. However, the resulting plasmid will loose its ampicilin resistance. In addition, The cloned DNA fragment will not be downstream to a promoter, and so the protein coded by this DNA won't be expressed.
Try again.

 
 































2. Wrong - technically you can perform the cloning. the location of the DNA fragment relative to the origin of replication is not important. What matters is its location relative to the promoter.

Try again.

 
 

































3. Right - technically you can perform the cloning. However, the resulting plasmid will loose its ampicilin resistance. In addition, The cloned DNA fragment will not be downstream to a promoter, and so the protein coded by this DNA won't be expressed.

Go to the next question.