Try again.

2. Wrong - the length of the DNA fragment is 345 nucleotides, but the last codon is a stop codon and does not encode for an amino acid.

Try again.

3. Right - the length of the DNA fragment is 345 nucleotides. The last codon is a stop codon and does not encode for an amino acid. Thus the protein encoded by this sequence comprises only 114 amino acids.
Including the stop codon of GOI in the plasmid may be crucial. Suppose you want to use a plasmid that encodes for a his-tag at the carboxy terminal of the protein (= at the 3' of GOI). In this case the presence of the stop codon will prevent the formation of the fusion protein. As opposed to that, if you wish to add a tag to the N terminus, the presence or absence of the stop codon, will not affect the formation of the fusion protein.

Go to the next question.

4. Wrong - the length of the DNA fragment is 345 nucleotides. The nature of the codons comprising the sequence does not affect the sequence length.

Try again.
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 

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