1 Wrong - The MCS will contain the HindIII and NcoI restriction sites, however the restriction site of EcoRI will be included in pQE60-GOI, since it is derived from pQE60.
Try again.






















































2 Wrong - The MCS will contain the HindIII and BamHI restriction sites (the BamHI site originates from pQE60). The XhoI restriction site is not lost. It originates from pQE60.

Try again.

























































3 Wrong - There is a restriction site of BglII in pQE60-GOI, that is driven from GOI.

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4  Right - There are indeed two restriction sites of NcoI in pQE60-GOI, that were driven from pGEM-GOI and from pQE60.

Go to the next question.
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 

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