1 Wrong - Cloning of pGEM-GOI into pQE60 will cause the lost of Ori from pGEM-GOI. However, though not mentioned in the illustration, every commercial plasmid, including pQE60 contains an Ori. Hence, the final plasmid, pQE60-GOI, will also contain an Ori.
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2 Wrong - There is only one restriction site of EcoRI in pQE60-GOI which originates from pQE60. Elements from the Lac promotor are present in pQE60 (click here to see them), and thus will be contained in pQE60-GOI.

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3 Wrong - Ampicillin is contained within pQE60-GOI (originating from pQE-60). There is a Stop codon in pQE60-GOI (the last codon in GOI [tag] is a stop codon).

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4  Right - The NcoI site within pQE-60 contains an ATG codon in frame after the RBS (ribosome binding site) and before the ATG of GOI. Thus a stretch of 12 nucleotides within the reading frame is created: ATGgga gga tcc

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