pQE-60
The bold sequence is described in more detail at the bottom of this page.

ctcgagaaatcataaaaaatttatttgctttgtgagcggataacaattataatagattca
attgtgagcggataacaatttcacacagaattcattaaagaggagaaattaaccatggga
ggatccagatctcatcaccatcaccatcactaagcttaattagctgagcttggactcctg
ttgatagatccagtaatgacctcagaactccatctggatttgttcagaacgctcggttgc
cgccgggcgttttttattggtgagaatccaagctagcttggcgagattttcaggagctaa
ggaagctaaaatggagaaaaaaatcactggatataccaccgttgatatatcccaatggca
tcgtaaagaacattttgaggcatttcagtcagttgctcaatgtacctataaccagaccgt
tcagctggatattacggcctttttaaagaccgtaaagaaaaataagcacaagttttatcc
ggcctttattcacattcttgcccgcctgatgaatgctcatccggaatttcgtatggcaat
gaaagacggtgagctggtgatatgggatagtgttcacccttgttacaccgttttccatga
gcaaactgaaacgttttcatcgctctggagtgaataccacgacgatttccggcagtttct
acacatatattcgcaagatgtggcgtgttacggtgaaaacctggcctatttccctaaagg
gtttattgagaatatgtttttcgtctcagccaatccctgggtgagtttcaccagttttga
tttaaacgtggccaatatggacaacttcttcgcccccgttttcaccatgcatgggcaaat
attatacgcaaggcgacaaggtgctgatgccgctggcgattcaggttcatcatgccgtct
gtgatggcttccatgtcggcagaatgcttaatgaattacaacagtactgcgatgagtggc
agggcggggcgtaatttttttaaggcagttattggtgcccttaaacgcctggggtaatga
ctctctagcttgaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgt
tttatctgttgtttgtcggtgaacgctctcctgagtaggacaaatccgccgctctagagc
tgcctcgcgcgtttcggtgatgacggtgaaaacctctgacacatgcagctcccggagacg
gtcacagcttgtctgtaagcggatgccgggagcagacaagcccgtcagggcgcgtcagcg
ggtgttggcgggtgtcggggcgcagccatgacccagtcacgtagcgatagcggagtgtat
actggcttaactatgcggcatcagagcagattgtactgagagtgcaccatatgcggtgtg
aaataccgcacagatgcgtaaggagaaaataccgcatcaggcgctcttccgcttcctcgc
tcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaagg
cggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaag
gccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctcc
gcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacag
gactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccga
ccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctc
atagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtg
tgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagt
ccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagca
gagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctaca
ctagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagag
ttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgca
agcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacgg
ggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaa
aaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagta
tatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcag
cgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacga
tacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcac
cggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtc
ctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagta
gttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcac
gctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacat
gatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaa
gtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactg
tcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgag
aatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgc
cacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactct
caaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgat
cttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatg
ccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttc
aatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgta
tttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacg
tctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggccct
ttcgtcttcac
 

ctcgagaaatcataaaaaatttatttgctttgtgagcggataacaattataatagattca
XhoI
attgtgagcggataacaatttcacacAgaattcattaaagaggagaaattaaccATGgga
                            EcoRI                    NcoI
ggatccagatctcatcaccatcaccatcactaagcttaattagctgagcttgGactcctg
BamHI BglII     Poly His       HindIII