1. Wrong - we use antibodies from different sources (e.g. rabbit and mouse) for the immunoprecipitation and for the immunoblotting. Later, during the immunoblotting, we use a secondary antibody which can only recognize the Fc domain of the primary antibody used in the previous step of the immunoblotting. Thus we can see that there is a difference in the expression of POI in different organs of the Centaur.
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2. Wrong.
 

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3. Right -

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