You have decided to take the whole cell lysates, obtained as previously described, and perform immunoprecipitation using anti-POI antibodies to increase the relative amounts of the POI protein in the different samples. You then perfomed immunoblotting using the enriched samples.
Here are your results


What conclusions can you draw from this experiment?

1. Immunoprecipitation using anti-Poi antibodies did not enrich the POI in our samples. Instead we see here the anti-POI antibodies themselves.
2. POI is expressed uniformly in all organs examined.
3. There is a gradient of expression: POI is expressed in higher levels in human organs, in lower levels in transition organs, and in horse organs it is not expressed at all or it is expressed in levels below the detection capability of this method.