Note:
the first two samples on the left of each figure (1.0 P and S) are samples
of the control GST-GFP system (expected size ~55 KDa) grown in 37oc
and induced by 1mM IPTG.
Scroll down to examine
all results.
|
Conditions
|
Coomassie Staining
|
Western Blot (anti-GST
ABs)
|
| Rosetta pLysS 2 hrs |
 |
 |
| Rosetta pLysS 4 hrs |
 |
 |
| Rosetta pLysS overnight |
 |
 |
.
Q5
Let's
focus on the overnight results, which are of higher quality (the sparation
is better).
Go
through the following observations and choose "True" or "False" for each
of them.
There
may be more than one "right" answer.
1. Higher
amounts of protein are observed when cultures are grown in 37oc.
2. Proteins
obtained following growth in RT are far more soluble than those obtained
following growth in 37oc.
3.
The relative amount of the full length product in RT
is higher than that in 37oc.
4.
Overnight exposure to 1mM IPTG in room temperature
yields no product.
Have
you examined all possibilities?
Let's examine the expression
of NS4B using RTS in-vitro translation.
Continue.