You chose the appropriate vector and inserted BHR1 into it. You then checked the insertion by restriction enzyme analysis. Now you perform transposition of your donor vector into DH10Bac bacterial cells () as illustrated below:

Four plates were obtained by you and your coleages:
Evaluate the following conclusions (choose between wrong  and possible).
1. In plate A all the bacteria incorporated the donor plasmid.
2. In plate B all the bacteria incorporated the donor plasmid.
3. In plate C all cells died because you did not add all the required antibiotics.
4. In plate D some of the bacteria apparently contain the BHR1 gene.