Preparing the PCR Product of the Gene of Interest
Flanked by the attB Recombination Sites

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    The designed primers should contain flanking regions with the attB recombination sequences, a ribosome binding site (Shine-Delgarno or Kozak) and a start codon ATG (if the gene is cloned into a vector without N-terminal fusion tag), followed by a sequence that has 18-24 bases homology with the gene's sequence.
    Under what circumstances will you get the translation of the attB recombination site?