Now that we learnt the necessary information about the two
plasmids involved in the cloning process (pGEM-GOI and pQE-60), it's high
time to use the CloneIt software.
Stop and wait for a shortexplanation
by the lecturer.
To open a new window showing pQE-60 in fasta format, click
To open another window showing CloneIt, click here.
If you closed the window showing pGEM-GOI in fasta
format, click here.
Now go to the CloneIt window and follow the instruction:
Three solutions are suggested. First we'll examine the second
to the next page.
Copy and paste the sequences of pGEM-GOI and pQE-60 to the
their appropriate places within the CloneIT window.
Use the plasmids pages to write the positions of the ATG
and cloning boxes in the appropriate places within the CloneIt window.
Scroll down to parameters and choose the option "just
use classical enzymes". Make sure that all the other options are not
Scroll further down and press the CloneIt button.