-
Very likely
The template that was used for the PCR reaction was not properly linearized,
and traces of this original vector have taken over the transformation.
To check this possibility, prepare plasmid preps from a few colonies and
check them after enzymatic restriction on agarose gels.
Check the other possibilities
-
Possible
check the plates by growing bacteria without resistance to antibiots.
Check the other possibilities
-
Wrong
The pEnter plasmid contains resistance to kanamycin only.
Check the other possibilities
-
Wrong
such mutations will allow too many colonies on the kanamycin plates, since
the vectors that did not replace the toxic
gene
with the ras gene will also grow. However, the vectors do not contain ampicylin
resistance.
Check the other possibilities
.