Ras Reaction
Positive Control
Negative Control
+ Kan
+ Amp
 

You have got colonies both on Ampicillin and Kanamycin plates of the Ras Reaction. What can be the possible explanation for this result? (Choose between wrong and possible)
Check ALL answers.
  1. The restriction of the original plasmid was insufficient, therefore it has been transformed to DH5a and grew on Amp plates as background noise in addition to the desired colonies containing Ras.
  2. The selective ampicillin plates contained too little antibiotics, therefore bacterial cells without plasmids grew on them.
  3. The few ras containing colonies that grew on the ampicillin plates resulted from a low-efficiency BP reaction.
  4. Mutation/s in the toxic gene ccdB made the bacteria viable, thus allowing bacterial growth on the ampicillin plates.
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