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Ras Reaction
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Positive Control
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Negative Control
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+ Kan
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+ Amp
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You have got colonies both on Ampicillin and Kanamycin plates
of the Ras Reaction. What can be the possible explanation for this result?
(Choose
between wrong and possible)
Check ALL answers.
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The restriction of the original
plasmid was insufficient, therefore it has been transformed to DH5a
and grew on Amp plates as background noise in addition to the desired colonies
containing Ras.
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The selective ampicillin
plates contained too little antibiotics, therefore bacterial cells without
plasmids grew on them.
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The few ras containing colonies
that grew on the ampicillin plates resulted from a low-efficiency BP reaction.
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Mutation/s in the toxic
gene ccdB made the bacteria viable, thus allowing bacterial growth
on the ampicillin plates.
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