^{The Hebrew University of Jerusalem
The Alexander Silberman Institute of Life Sciences}

Required Reading
How can I expand my search?

Textbooks in Harman Library

Companion websites

1. Gene expression technology (Series: Methods in Enzymology, Vol 185), San Diego : Academic Press, 1990
2. Recombinant DNA (Series: Methods in enzymology ; Vol 216 ) San Diego : Academic Press, 1992
3. Recombinant DNA (Series: Methods in enzymology ; Vol 217 ) San Diego : Academic Press, 1993
4. Recombinant DNA (Series: Methods in enzymology ; Vol 218 ) San Diego : Academic Press, 1993

 

 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 

1. Kamp, Roza Maria et al, Protein structure analysis : preparation, characterization, and microsequencing, Berlin, Springer-Verlag, 1997
2. Expression of recombinant genes in eukaryotic systems (Series: Methods in enzymology ; Vol 306 ) San Diego : Academic Press, 1999
3. Applications of chimeric genes and hybrid proteins Vol A, (Series: Methods in enzymology ; Vol 326 ) San Diego : Academic Press, 2000
4. Applications of chimeric genes and hybrid proteins Vol B, (Series: Methods in enzymology ; Vol 327 ) San Diego : Academic Press, 2000
5. Applications of chimeric genes and hybrid proteins Vol C, (Series: Methods in enzymology ; Vol 328 ) San Diego : Academic Press, 2000

1. Anatomy of an Expression Vector -

http://wolfson.huji.ac.il/purification/index.html   This site includes a plethora of information about purification strategies, Purification
                    of Recombinant Proteins, Cleavage of Recombinant Proteins, Chromatography, Protein Aggregation and Refolding,
                    Concentration, Desalting columns, Dialysis, Ultrafiltration, Inhibitors, DNA removal, Endotoxin removal, Detergents,
                    Bacterial Expression Systems, Eukaryotes Expression Systems, Choice of Buffers, Gel Electrophoresis, Proteomics Sample
                    Preparation, Gel Stain, Protein Quantitation, etc.
 

Protein Purification Strategies

AMERSHAM-BIOSCIENCES    The Recombinant Protein Handbook (pdf) (pdf-II)
AMERSHAM-BIOSCIENCES    Handbook Protein Purification (pdf)
AMERSHAM-BIOSCIENCES  Purifying Challenging Proteins: Membrane proteins, Multiprotein complexes and Inclusion bodies  (pdf)
BIO RAD                                       Sample Preparation Handbook (pdf)
CALBIOCHEM                             Calbiochem Inhibitor SourceBook (pdf)
MILLIPORE - AMICON             Ultrafiltration:Centrifuge Filter Devices (pdf-I)(pdf-II)
NOVAGEN                                    Preparation of Protein sample for SDS-PAGE Procedures and Tips - Article (pdf)
SIGMA                                          Useful pH Ranges of Selected Biological Buffers (pdf)
VIVASCIENCE                             Ultrafiltration Products Catalogue (pdf)

Jonasson P.,               Genetic Design for Facilitated Production and  Recovery of  Recombinant Proteins in E.Coli.
                                    Biotechnol.Appl.Biochem 2002, 35: 91-105(pdf)
Nilsson J.,                  Affinity Fusion Strategies for Detection, Purification and Immobilization of Recombinant Proteins.
                                    Protein Expr. and Purif. 1997, 11: 1-16   (pdf)
Swartz J.                    Advances in E.Coli Production of Therapeutic Proteins.
                                   Current Opinion in Biotechnology 2001, 12: 195-210  (pdf)
Sorensen H.,             Advanced genetic strategies for recombinant protein expression in Escherichia coli
                                  Journal of Biotechnology 115 (2005) 113–128  (pdf)
 

Ion Exchange Chromatography

AMERSHAM-BIOSCIENCES    Ion Exchange Cromatography and Chromatofusing Handbook (pdf)
 

Gel Filtration Chromatography

AMERSHAM-BIOSCIENCES    Gel Filtration Handbook (pdf)
 

Affinity Chromatography

AMERSHAM-BIOSCIENCES    Affinity Manual  (pdf)
AMERSHAM-BIOSCIENCES    Antibody  Purification Manual   (pdf)
AMERSHAM-BIOSCIENCES    GST fusion protein Handbook (pdf)
CLONTECH                                  BD TalonTM Metal Affinity Resins User Manual   (pdf)
NEW ENGLAND BIOLAB          pMAL protein Fusion and Purification System (pdf-I)
NOVAGEN                                    Preactivated Resins  (pdf)
NOVAGEN                                    pET purification manual  (pdf)
PIERCE                                         Batch and Spin Cup Methods for Affinity Purificatio of Proteins  (pdf)
PIERCE                                         Coupling different Functional Groups  (pdf)
QIAGEN                                        Ni-NTA purification manual  (pdf)
QIAGEN                                        Two-Step Affinity Purification System Handbook - For expressing, purifying, and detecting proteins
                                                        carrying a 6xHis and Strep-tag II   (pdf)
STRATAGENE                             CBP Calmodulin-Binding Peptide Affinity Tag System (pdf)

Nilsson J.,                  Affinity Fusion Strategies for Detection, Purification and Immobilization of Recombinant Proteins.
                                   Protein Expr. and Purif. (1997), 11: 1-16   (pdf)
Arnau J.,                    Current strategies for the use of affinity tags and tag removal for the purification of recombinant proteins
                                 Protein Expression and Purification 48 (2006) 1–13  (pdf)
Banki M.,                   Simple bioseparations using self-cleacing elastin-like polypeptide tags
                                  Nature Methods 2, 659, (2005)  (pdf)
Dyson M.,                  Production of soluble mammalian proteins in Escherichia coli: identification of protein features that correlate with
                                    successful expression   BMC Biotechnology (2004), 4:32(pdf)
Lichty J.,                    Comparison of affinity tags for protein purification
                                  Protein Expression and Purification 41 (2005) 98–105  (pdf)
Nallamsetty S.,           Solubility-enhancing proteins MBP and NusA play a passive role in the folding of their fusion partners
                                  Protein Expression and Purification 45 (2006) 175–182  (pdf)
Terpe K.,                    Overview of tag protein fusions: from molecular and biochemical fundamentals to commercial systems
                                  Appl Microbiol Biotechnol (2003) 60:523–533    (pdf)
 

Hydrophobic Exchange Chromatography

AMERSHAM-BIOSCIENCES    Hydrophobic Interaction and Reversed Phase Chromatography Principles and Methods  (pdf)
AMERSHAM-BIOSCIENCES    Hydrophobic Interaction Chromatography Manual  (pdf)
 

Other Purification Techniques

AMERSHAM-BIOSCIENCES    Chromatofusing Handbook (pdf)
AMERSHAM-BIOSCIENCES    Reverse Phase Chromatography Manual  (pdf)
AMERSHAM-BIOSCIENCES    Ion Exchange Cromatography and Chromatofusing Handbook (pdf)
VYDAC                                          The Handbook of Analysis and Purification of Peptides and Proteins by Reverse-Phase HPLC   (pdf)
 

Refolding and Aggregation problems

AMERSHAM-BIOSCIENCES  Purifying Challenging Proteins: Membrane proteins, Multiprotein complexes and Inclusion bodies  (pdf)
Altamirano M.,     Refolding Chromatography with Immobilized Mini-Chaperones.
                              PNAS 1997, 94: 3576-3578 (pdf)
Armstrong N.,      A New Protein Folding Screen...etc.
                             Protein Science 1999,  8: 1475-1483  (pdf)
Bondos S.,         Detection and prevention of protein aggregation before, during, and after purification
                              Analytical Biochemistry  (2003), 316 (2), 223-231   (pdf)

Chen G.,               Overexpression of a Glutamate Receptor (GluR2) ligand binding domain in E.Coli: Application of a novel protein                           
                              folding screen.  
                              Proc. Natl. Acad. Sci. USA Vol. 94, pp. 13431–13436, 1997    (pdf)

Eiler S.,                Overexpression, Purification, and Crystal Structure of Native ER alpha LBD.
                           Protein Expression and Purification (2001) 22, 165–173 (pdf)
Lee S.,                  Effects of solutes on solubilization and refolding of proteins from inclusion bodies with high hydrostatic pressure
                           Protein Science (2006), 15: 1-11   (pdf)
Lin L.,                  High-throughput identification of refolding conditions for LXRbeta without a functional assay
                           Protein Expression and Purification  (2006)  (pdf)
Machida S.,         Cycloamylose as an efficient artificial chaperone for protein refolding.
                             FEBS Letters 486 (2000) 131-135  (pdf)
Middelberg A.,    Preparative Protein Folding.
                            TRENDS in Biotechnology 2002, 20 (10): 437-443 (pdf)
Ming Li et al.,     In vitro protein refolding by chromatographic procedures.
                            Protein Expr. and Purif. 2004,33: 1-10  (pdf)
Shrodel A.,          Fusion tags and chaperone co-expression modulate both the solubility and the inclusion body features of the recombinant
                            CLIPB14 serine protease.  Journal of Biotechnology 120 (2005) 2–10   (pdf)
Tsumoto K.,        Practical Considerations in Refolding Proteins from Inclusion Bodies.
                            Protein Expr. and Purif. 2003,28: 1-8  (pdf)
Willis M.,            Investigation of protein refolding using a fractional factorial screen: A study of reagent effects and interactions
                          Protein Sci. 2005, 14: 1818-1826  (pdf)

Sample Preparation for X-Ray and NMR studies

Stevens R.,           Design of Hgh-Throughput Methods of Protein Production for Structural Biology.
                              Structure 2000, 8: R177-R185  (pdf)
Derewenda Z.,      The use of recombinant methods and molecular engineering in protein crystallization.
                              Methods (2004), 34: 354–363 (pdf)
Smyth D.,             REVIEW  Crystal structures of fusion proteins with large-affinity tags.
                             Protein Science (2003), 12:1313–1322 (pdf)
Eiler S.,                Overexpression, Purification, and Crystal Structure of Native ER alpha LBD
                             Protein Expression and Purification (2001) 22, 165–173(pdf)
Oganesyan N.     Effect of osmotic stress and heat shock in recombinant protein overexpression and crystallization.
                          Protein Expression and Purification 52 (2007) 280–285 (pdf)
Goldschmidt L.   Toward rational protein crystallization: A Web server for the design of crystallizable protein variants.
                            Protein Science (2007), 16:1569-1576  (pdf) 
Geerlof A. et.al.     The impact of protein characterization in structural proteomics
                                      Acta Cryst.(2006) D62: 1125-1136   (pdf) 

Membrane Proteins

AMERSHAM-BIOSCIENCES  Purifying Challenging Proteins: Membrane proteins, Multiprotein complexes and Inclusion bodies  (pdf)
CALBIOCHEM                           Biological Detergents. Guide for solubilization of membrane proteins and selecting tools for detergent
                                                         removal   (pdf)
CALBIOCHEM                             A guide to the properties and uses of detergents in Biology and Biochemistry (pdf)

Da-Neng Wang et al.       Practical aspects of overexpressing bacterial secondary membrane transporters for structural studies
                                          Biochimica et Biophysica Acta 1610 (2003) 23– 36   (pdf)
Said Eshaghi et al.           An efficient strategy for high-throughput expression screening of recombinant integral membrane proteins
                                        Protein Science, 14 (2005) 1-8 (pdf)

Textbooks

1. Deutscher, Murray P. editor, Guide to protein purification, San Diego : Academic Press, 1990
2. Matsudaira, Paul, A Practical guide to protein and peptide purification for microsequencing, San Diego : Academic Press, 1993
3. Scopes, Robert K., Protein purification : principles and practice, New York : Springer-Verlag, 1987
4. Marshak, Daniel R., Strategies for protein purification and characterization : a laboratory course manual, Plainview, New York : Cold Spring Harbor Laboratory Press, 1996
5. Ladisch, Michael R. editor, Protein purification : from molecular mechanisms to large-scale processes, Washington, D. C. : American Chemical Society, 1990
6. Conn, P. Michael, Handbook of proteomic methods, Totowa, NJ : Humana Press, 2003.
7. Liebler, Daniel C., Introduction to proteomics : tools for the new biology, Totowa, N.J. : Humana Press, 2002

1. Joel Janin, Shoshana J. Wodak, Protein modules and protein-protein interaction (part of the series ADVANCES IN PROTEIN CHEMISTRY), Amsterdam : Academic Press, 2003
2. Mount, David W., Bioinformatics : sequence and genome analysis, Cold Spring Harbor Laboratory Press, c2001
3. Misener, Stephen, Bioinformatics methods and protocols (Series: Methods in molecular biology Vol 132) Totowa, N. J. : Humana Press, 2000